Description
Short Description: The peptide corresponding to amino acids 556-688 that contains the bromodomain sequences of BRD1 (accession number NP_055392.1) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 21.5 kDa. Bromodomain-containing protein 1 (BRD1) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of several members that function in the regulation of transcriptional activation and chromatin remodeling. Recombinant BRD1 (556-688) can be used in binding assays and inhibitor screening